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Embryonic zebrafish primary cell culture for transfection ...

Oct 01, 2017 · Red fluorescent neuronal cells expressing the Purkinje cell specific reporter were observed for three days in culture showing characteristics such as relative large cell bodies and long neuronal processes (Fig. 6D-H). However, the typical extensive dendritic arbor covered with spines could not be observed likely because the tissue-specific ...

Upright and Inverted Microscopy | Theory and Applications ...

ibidi Solutions: All ibidi μ-Slides, μ-Dishes, and μ-Plates are designed for use with different types of inverted microscopes that include standard cell culture models, as well as complex microscopes, such as widefield fluorescence, confocal, spinning disk, and super-resolution. The ibidi μ-Slides, μ-Dishes, and μ-Plates are all equipped with a thin coverslip bottom with excellent ...

Immunocytochemistry and immunofluorescence protocol | Abcam

Blocking and immunostaining. Incubate cells with 1% BSA, 22.52 mg/mL glycine in PBST (PBS+ 0.1% Tween 20) for 30 min to block unspecific binding of the antibodies (alternative blocking solutions are 1% gelatin or 10% serum from the species the secondary antibody was raised in (typically goat serum, or donkey serum): see antibody datasheet for recommendations).

Protocol for the Preparation and Fluorescent ICC Staining ...

Immunocytochemistry Preparation & Fixation Protocol. Culture cells by adding 500 µL of culture media containing approximately 5000 cells to the wells of a cell culture plate containing gelatin-coated coverslips.; When cells have reached the desired density/age, remove the culture media from each well and wash twice with PBS.; Add 300-400 µL of 2-4% Formaldehyde Fixative Solution to each well ...

Lighting for Algae Cultures - Algae Research Supply

Fluorescent lamps emit across a wide spectrum. They do get warm and the lamps need to be replaced every year or so, which can add up in cost. If you are smart, you can capture some of the heat and use that to warm up your cultures (if they like to be warm). Figure-5: T-12 Fluorescent shop lamp

Fluorescence-activated Cell Sorting (FACS) | Sino Biological

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell.

Fluorescence In Situ Hybridization (FISH) protocol ...

Fluorescence in situ hybridization (FISH) is a technique that uses fluorescent probes which bind to special sites of the chromosome with a high degree of sequence complementarity to the probes. The fluorescent probes are nucleic acid labeled with fluorescent groups …

Observation of Fluorescent Proteins in Living Cells

Frequently, there is a need for fluorescent protein detection in mouse cell cultures, including embryonic stem cells or their differentiated derivatives, primary and transformed cells. Here, cells expressing green fluorescent protein-labeled proteins are observed using fluorescent microscopy.

Fluorescence Microscopy | Solutions | Leica Microsystems

Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Fluorescence is a form of luminescence that through microscopy allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ...

How to Take Quality Microscopy Pictures – ScienCell ...

When it comes to primary cell culture, a picture is worth a thousand words.A quality image can convey information on cell morphology, health, culture purity and density. Different types of 2-dimensional microscopy techniques such as phase contrast, relief contrast, and fluorescence microcopy can provide invaluable insight into your cultures when used concurrently.

Cell Dissociation with Trypsin | Mechanism in cell culture ...

Remove and discard the culture media from flask Gently rinse the cells with balanced salt solution without Ca +2 and Mg +2 ions and remove the solution. Firmly adherent cells could also be washed with tryspin solution. However, for sensitive cells balanced salt solution is preferred.

What is the impact of phenol red (culture medium component ...

I try to quantify microparticles released by neutrophils in the supernatant culture by flow cytometry. MP were stained with Annexin V - FITC. The MP were diluted in RPMI with phenol red.

Immunofluorescence Protocol (IF Protocol) | Sino Biological

Immunofluorescence is one of the widely used techniques in modern biology and medicine, and it is developed by Coons et al. (1950), and it is a combination of immunofluorescence technique and morphological technology to develop immune fluorescent cells (or tissue).

Culture, Yeast with Direct Fluorescent KOH | Test Detail ...

Culture, Yeast with Direct Fluorescent KOH - The significance of a yeast isolate varies with the organism identification, source, and clinical symptoms manifested.

3D Cell Culture and Fluorescence Imaging | Lab Manager

May 07, 2014 · 3D Cell Culture and Fluorescence Imaging Assays using immortalized cell lines and plastic labware have been used for decades as an in-vitro mimic of how a putative drug may work in the human body. May 7, 2014

Research and Development - Laboratory for Fluorescence ...

Exploiting natural differences in expression levels of fluorescent proteins among cells in culture to separate the contribution of the background fluorescence from the proteins and to quantify FRET efficiencies. Previous developments: Lifetime resolved medical endoscopy. Full field fluorescence lifetime-resolved imaging at video-rate.

Protocol for the Preparation and Fluorescent ICC Staining ...

Immunocytochemistry Preparation & Fixation Protocol. Culture cells by adding 500 µL of culture media containing approximately 5000 cells to the wells of a cell culture plate containing gelatin-coated coverslips.; When cells have reached the desired density/age, remove the culture media from each well and wash twice with PBS.; Add 300-400 µL of 2-4% Formaldehyde Fixative Solution to each well ...

Which Plates Should I Choose for Fluorescence and ...

Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the experiment. ... For multiplexed luminescent and fluorescent assays, using a white ...

Fluorescence activated cell sorting of live cells | Abcam

Fluorescence activated cell sorting (FACS) of live cells separates a population of cells into sub-populations based on fluorescent labeling. Sorting involves more complex mechanisms in the flow cytometer than a non-sorting analysis.

Fluorescent Cell Stains for Organelles & Cellular ...

Cell populations can be labeled with different fluorescent colors for identification after mixing. When live cells are cultured after staining, the dyes are internalized over time by endocytosis. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.

Fluorogenic Media - Microbiological Culture Media | Sigma ...

Culture media for the simple and fast detection of E. coli or other organisms using fluorescence substrates. E. coli , for example, can be detected using the MUG (glucuronidase) test and confirmed with a positive indole test. ß-D-glucoronidase, which is produced by E. coli , cleaves 4-Methylumbelliferyl-ß-D-glucuronide to 4-methylumbelliferone and glucuronide.

Lighting for Algae Cultures - Algae Research Supply

Fluorescent lamps emit across a wide spectrum. They do get warm and the lamps need to be replaced every year or so, which can add up in cost. If you are smart, you can capture some of the heat and use that to warm up your cultures (if they like to be warm). Figure-5: T-12 Fluorescent shop lamp

Cell Lines and Cultures | For Detection and Diagnosis | Quidel

Cell lines are available in shell vial or multi-well plate format for centrifuged-enhanced, rapid viral detection; in convenient ReadyCells ® format for on-demand culture; and in tube format for conventional culture. In addition, Quidel also manufactures a full menu of media, supplements, detection reagents, controls, and collection and ...